Issue 09/2013 Art. No. 7001-0229 Operating Manual APT.line™ CB (E6) CO2 - Incubators CO2 – Incubators with O2 control with sterilizable NDIR s
CB (E6) 09/2013 Page 10/144 Dear customer, For the correct operation of the CO2 incubator CB, it is important that you read this operatin
CB (E6) 09/2013 Page 100/144 15.8 Stands 15.8.1 Stacking stand (option) We recommend not stacking CB incubators directly on top of one an
CB (E6) 09/2013 Page 101/144 16. Reference measurements Reference measurements of the temperature, CO2, and O2 (unit with O2 control) can
CB (E6) 09/2013 Page 102/144 16.1.2 Measuring CO2 directly via chemical indicator tubes This is a common “do-it-yourself” test for many us
CB (E6) 09/2013 Page 103/144 16.1.3 Measuring CO2 directly with an electronic infrared measuring device The easiest way of measuring the C
CB (E6) 09/2013 Page 104/144 • Reduce the number of personnel and their movements in the lab by careful positioning all relevant equipment
CB (E6) 09/2013 Page 105/144 Condensation prevention • Condensation in the inner chamber represents a particular risk of contamination. Th
CB (E6) 09/2013 Page 106/144 • Regularly use the hot air sterilization function (chap. 18.3) following cleaning. The shelves and the empti
CB (E6) 09/2013 Page 107/144 18. Cleaning, decontamination / disinfection, and sterilization DANGER Electrical hazard. Danger of deat
CB (E6) 09/2013 Page 108/144 Soapsuds may contain chlorides and must therefore NOT be used for cleaning. With every decontamination met
CB (E6) 09/2013 Page 109/144 In case of contamination of the interior by biologically or chemically hazardous material, there are two possi
CB (E6) 09/2013 Page 11/144 WARNING Indicates a potentially hazardous situation which, if not avoided, could result in death or serious (i
CB (E6) 09/2013 Page 110/144 18.3 Hot-air sterilization at 180 °C / 356 °F The very first sterilization after operation may cause an odor
CB (E6) 09/2013 Page 111/144 18.3.2 Performing a hot-air sterilization Before carrying out the first hot-air sterilization, remove any pr
CB (E6) 09/2013 Page 112/144 Fixed value 08.03.2013 05:05:06 Submenu “Start hot-air sterilization”. This is a security question. Make sure
CB (E6) 09/2013 Page 113/144 Opening the outer door during sterilization leads to aborting the automatic sterilization cycle. CAUTION Inte
CB (E6) 09/2013 Page 114/144 This symbol on the controller display indicates that the hot-air sterilization was aborted. The interior may
CB (E6) 09/2013 Page 115/144 Aborting sterilization after less than 4 hours When aborting the sterilization prematurely, it may be that the
CB (E6) 09/2013 Page 116/144 Therefore, without using the stacking stand or the stacking adapter, safe incubation is not possible during st
CB (E6) 09/2013 Page 117/144 19.3 Checking the humidity system fan The user should regularly perform the following checks: Hold a sheet of
CB (E6) 09/2013 Page 118/144 20. Disposal 20.1 Disposal of the transport packing 20.1.1 Outer unit packing Packing element Material Disp
CB (E6) 09/2013 Page 119/144 20.2 Decommissioning • Turn off the unit at the main power switch and disconnect it from the power supply (p
CB (E6) 09/2013 Page 12/144 Mandatory action signs Mandatory regulation Read operating instructions Disconnect the power plug Lift wi
CB (E6) 09/2013 Page 120/144 • Prior to handing the unit over to a recycling company, it is the user’s responsibility that it is free fro
CB (E6) 09/2013 Page 121/144 Certified companies disassemble waste (used) BINDER equipment in primary substances for recycling according to
CB (E6) 09/2013 Page 122/144 21. Troubleshooting Only qualified service personnel authorized by BINDER must perform repair. Repaired uni
CB (E6) 09/2013 Page 123/144 Fault description / indication Possible cause Required measures Too low temperature. Having reached the setpo
CB (E6) 09/2013 Page 124/144 Note: The recovery times of the gas concentrations inside the chamber following door opening, which are indica
CB (E6) 09/2013 Page 125/144 Fault description / indication Possible cause Required measures Low N2 outlet pressure (< 0.3 bar) (unit
CB (E6) 09/2013 Page 126/144 Fault description / indication Possible cause Required measures Too low CO2 concentration. Having reached the
CB (E6) 09/2013 Page 127/144 21.4 Sterilization Fault description / indication Possible cause Required measures The sterilization is runni
CB (E6) 09/2013 Page 128/144 Fault description / indication Possible cause Required measures Condensation on the divided inner glass door,
CB (E6) 09/2013 Page 129/144 21.6 Controller Fault description / indication Possible cause Required measures The controller returns to the
CB (E6) 09/2013 Page 13/144 CO2 -Incubator CB CO2 -Incubator CB-UL Figure 1: Position of labels on the unit Keep safety labels complet
CB (E6) 09/2013 Page 130/144 22. Technical description 22.1 Factory calibration and adjustment This unit was calibrated and adjusted in t
CB (E6) 09/2013 Page 131/144 Do NOT place samples outside this usable volume. Do NOT load this volume by more than half to enable sufficie
CB (E6) 09/2013 Page 132/144 Unit size 60 160 220 Humidity data Humidity % r.H. 90 to 95 90 to 95 90 to 95 O2 Data O2 range vol.-% O2 0.2
CB (E6) 09/2013 Page 133/144 22.5 Equipment and Options To operate the CO2 incubator, use only original BINDER accessories or accessories
CB (E6) 09/2013 Page 134/144 Options / accessories (continued) Data Logger Kit T 220 Qualification folder Calibration of temperature includ
CB (E6) 09/2013 Page 135/144 Description Art. no. Controller T 4.12 Temperature sensor Pt 100 straight (door heating) 5002-0021 Temperatu
CB (E6) 09/2013 Page 136/144 22.9 Dimensions CB 60 (Dimensions in mm)
CB (E6) 09/2013 Page 137/144 22.10 Dimensions CB 160 (Dimensions in mm)
CB (E6) 09/2013 Page 138/144 22.11 Dimensions CB 220 (Dimensions in mm)
CB (E6) 09/2013 Page 139/144 23. Contamination clearance certificate 23.1 For units located outside North America and Central America Dec
CB (E6) 09/2013 Page 14/144 1.4 Type plate Position of type plate: left unit side (seen from front), at the bottom in the middle Figure 2
CB (E6) 09/2013 Page 140/144 3.3 Measures to be taken in case of skin contact or release into the atmosphere / Maßnahmen bei Personenkonta
CB (E6) 09/2013 Page 141/144 We hereby declare that the following measures have been taken / Wir erklären, dass folgende Maßnahmen getroffe
CB (E6) 09/2013 Page 142/144 23.2 For units in North America and Central America Product Return Authorization Request Please complete this
CB (E6) 09/2013 Page 143/144 Customer (End User) Decontamination Declaration Health and Hazard Safety declaration To protect the health of
CB (E6) 09/2013 Page 144/144 4. Declaration of Decontamination For toxic, radioactive, biologically and chemically harmful or hazardous s
CB (E6) 09/2013 Page 15/144 1.5 General safety instructions on installing and operating the CO2 incubator With regard to operating the CO2
CB (E6) 09/2013 Page 16/144 CAUTION The glass doors and the inner chamber will become hot during operation. Danger of burning. ∅ Do NOT
CB (E6) 09/2013 Page 17/144 Unit with O2 control: Notes on handling oxygen (O2) Oxygen (O2) is colorless and almost odorless and therefore
CB (E6) 09/2013 Page 18/144 1.7 Precautions when handling gas cylinders General information for safe handling of gas cylinders: • Store
CB (E6) 09/2013 Page 19/144 2. Unit description The CO2 incubators CB are equipped with a multifunctional microprocessor display controll
CB (E6) 09/2013 Page 2/144 EG – declaration of conformity EG – KONFORMITÄTSERKLÄRUNG EC - DECLARATION OF CONFORMITY CE - DECLARATION DE C
CB (E6) 09/2013 Page 20/144 2.1 Unit overview Instrument panel with microprocessor controller T4.12 and USB interface Door handle U
CB (E6) 09/2013 Page 21/144 2.3 Inner chamber Figure 5: CB 160 with O2 control and options FRO NT(A) (I) (J) (K) (L) (B) (C) (D) (E) (
CB (E6) 09/2013 Page 22/144 Figure 6: CB 160 / CB 220 with O2 control and options (A) Instrument panel with microprocessor controller T
CB (E6) 09/2013 Page 23/144 2.4 Control panel on the rear of the unit (1) (2) (3) (6) (4) (5) (7) (8) (9) (10) Figure 7: Re
CB (E6) 09/2013 Page 24/144 3. Completeness of delivery, transportation, storage, and installation 3.1 Unpacking, and checking equipment
CB (E6) 09/2013 Page 25/144 CAUTION Sliding or tilting of the unit. Damage to the unit. Risk of injury by lifting heavy loads. Trans
CB (E6) 09/2013 Page 26/144 CAUTION Danger of overheating. Damage to the unit. ∅ Do NOT set up units in non-ventilated recesses. Ensu
CB (E6) 09/2013 Page 27/144 Notes on handling carbon dioxide (CO2) Carbon dioxide (CO2) in high concentrations is hazardous to health. It i
CB (E6) 09/2013 Page 28/144 General information for safe handling of oxygen: • Make sure training of personnel on hazards of oxygen enric
CB (E6) 09/2013 Page 29/144 4. Installation and connections 4.1 Shelves You can put the shelves in different positions at the line of cha
CB (E6) 09/2013 Page 3/144 Die oben beschriebenen Produkte sind konform mit folgenden harmonisierten Normen: The products described above a
CB (E6) 09/2013 Page 30/144 • We recommend cleaning and refilling the pans 2 to 3 times a week. For evacuation, remove the Permadry™ water
CB (E6) 09/2013 Page 31/144 4.3.1 Connection of the CO2 gas cylinder Carbon dioxide (CO2) in high concentrations is hazardous to health. I
CB (E6) 09/2013 Page 32/144 The recovery times of the gas concentrations inside the chamber after opening the door are indicated in the te
CB (E6) 09/2013 Page 33/144 Establishing the connection to the incubator Connect the supplied gas hose (internal diameter 6 mm / 0.24 inche
CB (E6) 09/2013 Page 34/144 CAUTION Excessive outlet pressure > 2.5 bar / 36 psi. Damage to the unit. ∅ The outlet pressure must NOT e
CB (E6) 09/2013 Page 35/144 Remove the rubber cover (b) by pulling it off. Unit rear Figure 12: Connecting the hose lead to the gas cyli
CB (E6) 09/2013 Page 36/144 4.4 Electrical connection The CO2 incubator comes with an IEC connector plug. Model Art. No. (x = 0 or 1) Powe
CB (E6) 09/2013 Page 37/144 4.5 Handling and aligning the multiple-divided inner glass door, gas proof (optional equipment) Rubber prof
CB (E6) 09/2013 Page 38/144 5. Start up After connecting the supply lines (chap. 4.4) turn on the chamber by the main power switch. The
CB (E6) 09/2013 Page 39/144 6. Functional overview of the T4.12 chamber controller The T4.12 chamber controller controls the following val
CB (E6) 09/2013 Page 4/144 D-78532 Tuttlingen, 20.07.2012 BINDER GmbH P. M. Binder Geschäftsführender Gesellschafter Managing Director D
CB (E6) 09/2013 Page 40/144 6.1 Menu structure Fixed value 08.03.2013 05:05:06 Initial view (sample values, unit with O2 control). Pres
CB (E6) 09/2013 Page 41/144 Fixed value 08.03.2013 05:05:06 General menu (next page) ..\ Menu Settings Measurement chart Interior socket
CB (E6) 09/2013 Page 42/144 Measurement chart Graphical display of the measured values, chap. 13 Active alarms Alarm settings, chap. 11.5
CB (E6) 09/2013 Page 43/144 6.2 Operating modes In the “control off” mode (chap. 6.2.1), the controller is non-functional and displays
CB (E6) 09/2013 Page 44/144 Control off 08.03.2013 05:05:06 General menu. The controller mode “Fixed value” or “Control off” is indicated
CB (E6) 09/2013 Page 45/144 6.3 Deactivating the O2 control and O2/N2 pressure alarms (unit with O2 control) 6.3.1 Required gas supply of
CB (E6) 09/2013 Page 46/144 6.3.2 Activating / deactivating the O2 control and O2 / N2 pressure alarms To activate or deactivate the O2 co
CB (E6) 09/2013 Page 47/144 6.4 Humidity control of the Permadry™ system The Permadry™ system with its 2-pan water system guarantees a max
CB (E6) 09/2013 Page 48/144 6.5 Turning on / off the interior socket voltage (with optional interior socket) For chambers equipped with th
CB (E6) 09/2013 Page 49/144 6.7 Information You access chamber information like the chamber type, serial no., firmware version etc. To dis
CB (E6) 09/2013 Page 5/144 Product registration
CB (E6) 09/2013 Page 50/144 7. Setpoint entry 7.1 Setting ranges Temperature 7 °C / 12.6 °F above ambient up to 60 °C / 140 °F CO2 0 vol
CB (E6) 09/2013 Page 51/144 WARNING High concentration of O2 (> 21 % O2). Fire and explosion hazard through contact of combustible ma
CB (E6) 09/2013 Page 52/144 7.3 Entering the setpoints via “quick menu” To enter setpoints via quick menu, go to Quick menu. Fixed value
CB (E6) 09/2013 Page 53/144 Setting the CO2 concentration To enter the CO2 setpoint, go to Quick menu > CO2 setpoint Fixed value 08.03.2
CB (E6) 09/2013 Page 54/144 7.4 Entering the setpoints via general menu To enter setpoints via general menu, go to Menu > Setpoints Fix
CB (E6) 09/2013 Page 55/144 Setting the CO2 concentration To enter the CO2 setpoint, go to Menu > Setpoints > CO2 setpoint Fixed valu
CB (E6) 09/2013 Page 56/144 8. Key lock The key lock function serves to block the access to the controller. When the “key lock” function
CB (E6) 09/2013 Page 57/144 8.1 Directly activating the key lock To directly activate the key lock, go to User > Key lock > Key loc
CB (E6) 09/2013 Page 58/144 Under “Waiting time [min]” you can enter the waiting time, after which the key lock will be automatically activ
CB (E6) 09/2013 Page 59/144 This symbol on the controller displays indicates that the “key lock” function is activated. 8.3 Changing the
CB (E6) 09/2013 Page 6/144 Contents EG – declaration of conformity ...
CB (E6) 09/2013 Page 60/144 9. General controller settings In the "Settings" submenu, you can enter the date and time, select th
CB (E6) 09/2013 Page 61/144 9.1 Setup wizard The setup wizard will guide you sequentially through the important menus to configure your ch
CB (E6) 09/2013 Page 62/144 Function “Set date” Fixed value 08.03.2013 05:05:06 Entry menu “Select date”. The current date is shown. If it
CB (E6) 09/2013 Page 63/144 9.3 Selecting the menu language of the T4.12 controller The T4.12 chamber controller communicates via a compre
CB (E6) 09/2013 Page 64/144 9.5 Changing the temperature unit To select the temperature unit, go to Menu > Settings > Temperature un
CB (E6) 09/2013 Page 65/144 9.7 Factory reset The “factory reset” function allows resetting the controller configuration to the factory se
CB (E6) 09/2013 Page 66/144 Fixed value 08.03.2013 05:05:06 Submenu “Network settings” (next page). ..\ Network settings DNS2
CB (E6) 09/2013 Page 67/144 Enter the IP address: Fixed value 08.03.2013 05:05:06 Entry menu “IP address” (example value) Enter the desir
CB (E6) 09/2013 Page 68/144 Enter the network name: Fixed value 08.03.2013 05:05:06 Entry menu “Network name”. Enter the desired network n
CB (E6) 09/2013 Page 69/144 9.9 Display of the network configuration To access the overview of the complete network configuration, go to
CB (E6) 09/2013 Page 7/144 6.3 Deactivating the O2 control and O2/N2 pressure alarms (unit with O2 control) ...
CB (E6) 09/2013 Page 70/144 9.11 RS 422 address (with optional RS 422 interface) For chambers equipped with the optional RS 422 interface,
CB (E6) 09/2013 Page 71/144 9.12 Display and entry of the device configuration – for service purpose Information about the chamber, such a
CB (E6) 09/2013 Page 72/144 10. Data transfer via USB interface The USB port is located in the instrument box. To access the submenus for
CB (E6) 09/2013 Page 73/144 If no USB device has been connected, the message “Insert USB device” is displayed. It disappears after inserti
CB (E6) 09/2013 Page 74/144 11. Notifications and Alarms 11.1 Notifications overview Icon Signification Icon Signification Fixed value o
CB (E6) 09/2013 Page 75/144 Icon Alarm message Signification Humidity range After the humidity was situated within the defined tolerance r
CB (E6) 09/2013 Page 76/144 11.3 Alarm status An alarm message can appear in 3 different states: “Set” • Active alarm. • The correspon
CB (E6) 09/2013 Page 77/144 11.4 Confirming an active “set” alarm Fixed value 08.03.2013 05:05:06 Initial view with overtemperature safet
CB (E6) 09/2013 Page 78/144 11.5 Alarm configuration and overview To access the alarm lists and configuration menu, go to Menu > Alarms
CB (E6) 09/2013 Page 79/144 11.5.2 History – list of all alarms To access the overview list of all alarms, go to Menu > Alarms > His
CB (E6) 09/2013 Page 8/144 14. TEMPERATURE SAFETY DEVICES ... 91 14.1 Ove
CB (E6) 09/2013 Page 80/144 11.5.3 Activating, deactivating, and testing the alarm buzzer Alarm buzzer test To access the alarm buzzer fun
CB (E6) 09/2013 Page 81/144 11.5.5 Setting the delay time after opening the door To enter the delay time for the door open alarm, go to Me
CB (E6) 09/2013 Page 82/144 Temperature First you can enter the desired value of the temperature tolerance range: Enter tolerance range 08.
CB (E6) 09/2013 Page 83/144 Then you can enter the delay time for the CO2 tolerance range alarm: Enter alarm delay [min] 08.03.2013 05:05:
CB (E6) 09/2013 Page 84/144 Then you can enter the delay time for the humidity tolerance range alarm: Enter alarm delay [min] 08.03.2013 0
CB (E6) 09/2013 Page 85/144 Service settings Fixed value 08.03.2013 05:05:06 Submenu “Service settings”. Access to the following submenus
CB (E6) 09/2013 Page 86/144 11.6 Zero-voltage relay alarm output The CO2 incubator is equipped with a zero-voltage relay output which perm
CB (E6) 09/2013 Page 87/144 12. Event list The “Event list” displays status information and errors of the current day. You can also access
CB (E6) 09/2013 Page 88/144 13. Graphical display of the measured values To access the graphical display, go to Menu > Measurement char
CB (E6) 09/2013 Page 89/144 13.2 Defining the display range To define the display range, go to Menu > Measurement chart > Settings &
CB (E6) 09/2013 Page 9/144 21. TROUBLESHOOTING ... 122
CB (E6) 09/2013 Page 90/144 13.3 Selecting the parameters Here you can select the parameters, which shall be displayed graphically. To sel
CB (E6) 09/2013 Page 91/144 14. Temperature safety devices 14.1 Overtemperature protective device (class 1) The CO2 incubator is equippe
CB (E6) 09/2013 Page 92/144 14.2.2 Setting the safety controller To display and to change the current safety controller settings in the “s
CB (E6) 09/2013 Page 93/144 Fixed value 08.03.2013 05:05:06 Entry menu “Safety controller overtemperature”. Enter the desired value with t
CB (E6) 09/2013 Page 94/144 Fixed value 08.03.2013 05:05:06 Overview display with “Offset” mode (example values) View Setpoint Actual Ch
CB (E6) 09/2013 Page 95/144 15. Options 15.1 Communication software APT-COM™ 3 DataControlSystem (option) The unit is regularly equipped
CB (E6) 09/2013 Page 96/144 When operating a CB with silicon access ports, both silicon plugs must tightly close the access ports. If the p
CB (E6) 09/2013 Page 97/144 Heat emission of electrical devices connected inside the CO2 incubator may modify the temperature range. The m
CB (E6) 09/2013 Page 98/144 15.5 Analog outputs for temperature and CO2 (option) With this option, the CO2 incubator is equipped with anal
CB (E6) 09/2013 Page 99/144 On unit rear: Exterior Lemo socket (8) on the rear of the unit Interior: LEMO socket (B) located at the up
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